… Indeed, it is a realistic possibility that the complete genome of a filamentous fungus will
be determined at the nucleotide level, with Aspergillus nidulans as the strongest …

… groups of fungi can be transformed, and the genetic manipulation of … In this chapter, we focus
on five filamentous fungi comprising … or agriculture, ie Aspergillus niger, Aspergillus oryzae, …

… , which contains a fungal expression cassette with the Aspergillus nidulans gpdA promoter
(Punt et al. 1992), a multiple cloning site (MCS), and the A. nidulans trpC terminator [trpC(t)] (…

… repair (HDR) occurs (15). We previously achieved efficient genetic engineering for mutagenesis
and gene … In the present study, we developed efficient genetic engineering techniques …

… tumefaciens is used as a vector for gene transfer in various filamentous fungi. AMT was first
… available gene editing methods and transformation efficiency are low, but filamentous fungi …

… of filamentous fungi are highly dependent on effective genetic manipulation. However, the
development of gene … AMA1: (a sequence based on its origin from Aspergillus nidulans, …

… Efficient strain engineering requires genetically tractable hosts. Several methods have
been established to genetically engineer filamentous fungi such as protoplast transformation, …

… , is still widely used especially in the model filamentous fungi-including Aspergillus nidulans
[3]. Empirical modifications of the technique have, in general, either sought to improve …

… Filamentous fungi occupy different niches in natural habitats and play a critical role in the …
, elucidation of fungal gene functions by high-throughput analyses requires efficient gene-…

… methods, gene complementation strategies and biotechnological application of gene
complementation for filamentous fungi. In addition, we discuss the challenges to current gene …